| Clone | AX.14 |
| Isotype | IgG1 |
| ProductType | Single-ColorReagent |
| Units | 100Tests |
| Host | Mouse |
| Speciesreactivity | Human |
| Application | FlowCytometry |
BackgroundTheCD103antigenisa160-130-105kDamoleculewhichisexpressedonmucosa-associatedTlymphocytesandactivatedcellsandonsubsetofTGFbeta-1cells.ThishumanintraepitheliallymphocyteMarkeristheEintegrinchainassociatedwiththeb7subunit.CD103antibodystainsafewbonemarrowcellsand,rarely,peripheralbloodlymphocytes.CD103isexpressedby0.5to2%ofrestinglymphocytesinPeripheralbloodandlymphoidorgans.
Synonyms:CD103PE
Product
ProductForm:RPE
Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazideand0.2%carrierprotein
PurificationMethod:ProteinA/GChromatography
Concentration:Titeredforflowcytometry
ApplicationsPBMC:Add10µlofMAB/106PBMCin100µlPBS.Mixgentlyandincubatefor15minutesat2°to8°C.WashtwicewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.WHOLEBLOOD:Add10µlofMAB/100µlofwholeblood.Mixgentlyandincubatefor15minutesatroomtemperature(20°C).Lysethewholeblood.WashoncewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.SeeinstrumentmanufacturersinstructionsforLysedWholeBloodandImmunofluorescenceanalysiswithaflowcytometerormicroscope.ALLOPHYCOCYANIN:(APC)conjugatesareanalyzedinmulti-colorflowcytometrywithinstrumentsequippedwithasecondlaserandproperfilters.Laserexcitationisat633nmwithaHeliumNeon(HeNe)laserora600-640nm(633nm)rangeforaDyelaser.Peakfluorescenceemissionisat660nm.
FunctionalAnalysis:FlowCytometryStaining
StorageProductshouldbestoredat4-8ºC.DONOTFREEZE
ProductStABIlity:Reagentsarestablefortheperiodshownontheviallabelwhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.CepekK.,WongD.BrennerM.,andSpringerT.,LeukocyteTypingVval.Gp1666-67(1995).
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:P38570
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
