BackgroundRap1A is a 21 kDa small GTP-binding protein that antagonizes the Ras transforming activity through tight binding to Ras-GAP.It interferes with Ras-dependent Raf1 activation by inhibiting binding of Ras to the cysteine-rich region of Raf1.This protein is localized in the endoplasmic reticulum, late endosomes, and lysosomes, indicating its potential role in the regulation of intracellular protein degradation. Rap1a contains a C-terminal CLLL motif for post-translational geranylgeranylation and a single site for phosphorylation by protein kinase A. Ras proteins provide a crucial link in the intracellular signalling cascades. Ras proteins are members of the small G protein family. These proteins are characterized by a molecular weight between 20 and 30 kDa, the ability to bind GTP or GDP and a low intrinsic GTPase activity. These proteins relay signals from the plasma membrane activated by receptor tyrosine kinases to the nucleus via a network of serine/threonine kinases. Ras proteins belong to a family of approximately 40 mammalian and 60 non-mammalian proteins. Ras proteins were first discovered as the hyperactive products of mutant Ras genes which promote cancer by disrupting the normal controls on cell proliferation and differentiation; about 30% of human cancers have mutations in a Ras gene.

Synonyms: Rap 1A Recombinant G-Protein, G protein

Source

Immunogen: Recombinant GST protein from E. Coli

Product

Product Form: Unconjugated

Formulation: Provided in 50mM Tris (pH 7.5) solution containing 150 mM NaCl, 10 mM MgCl2, 1 mM DTT, 10% glycerol

Concentration: See vial for concentration

ApplicationsRap1A protein is provided as a functional G-protein and can be used as a Western blot control for antibodies to Rap1A protein or in assays to study to protein-protein interactions of Rap1A and related proteins with regulatory proteins.

Functional Analysis: Western Blotting

StorageProduct should be stored at -70ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship on dry ice, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Takai, Y., et al. 'Small GTP-binding proteins.' Physiol. Rev. 2001, 81, 153-208 2. McPhee, I., et al. 'Use of an activation-specific probe to show that Rap1A and Rap1B display different sensitivities to activation by forskolin in rat1 cells.' FEBS Lett. 2000, 477, 213-218 3. Herrmann, C., et al. 'Differential interation of the ras family GTP-binding proteins H-Ras, Rap1A and R-Ras with the putative effector molecules Raf kinase and Ral-guanine nucleotide exchange factor.' J. Biol. Chem. 1996, 271, 6794-6800.

Protein Reference(s)

Database Name: SwissProt

Accession number: P62834

Species Accession: Human

Safety Datasheet(s) for this product:NM_Glycerol/wp-content/uploads/SDS/SDS Antibodies with glycerol Nordic-MUbio.pdf