BackgroundMonoclonal antibody 1304 detects CD54 (ICAM-1) and is useful for the detection of activated endothelial cells and other activated haematopoietic cells. The antibody inhibits the binding of natural killer (NK) cells to target cells (K562).Intercellular adhesion molecule (ICAM) 1 or CD54 was initially identified with monoclonal antibody RR1/1 as a widely distributed, cytokine-inducible counter-receptor for lymphocyte-function-associated antigen (LFA) 1 (CD11a/CD18). Granulomas (macrophages/epitheloid cells) in inflamed colon from Crohn's disease and follicular dendritic cells in the tonsil from chronic tonsillitis are strongly positive for CD54 antigen. Also, CD54 is found on activated CD56+/CD3- NK cells. Human CD54, the antibody detects an epitope in domain 1 of the 95 kDa transmembrane protein..

Source

Immunogen: Cultivated human endothelial cells

ProductAffinity purified, lyophilized antibody. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.

Purification Method: Affinity purified, lyophilized antibody. Reconstitute by adding 0.5ml distilled water. This stock solution contains 0.2mg/ml IgG, phosphate buffered saline pH 7.2 (PBS), 10mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01% thimerosal as a preservative.

Concentration: 0.1 mg lyophilized

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human

ApplicationsIHC(C, P)

Incubation Time: 60 min at RT or 18 hr at 2-8°C

Working Concentration: (lyophilisate) 2 µg/ml (IHC-P)

Pre-Treatment: Formaldehyde-fixed tissue slices are preferably pre-treated with proteinase K.

Positive Control: Human Tonsil

Storage2-8°C

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Albelda S.M., and Buck C.A. (1990) Integrins and other cell adhesion molecules. FASEB J. 4; 2868-2880. 2. Freudenthal P.S., and Steinmann R.M. (1990) The distinct surface of human blood dendritic cells, as observed after an improved isolation method. PNAS 87; 7698-7702. 3. Klickstein L.B., and Springer T.A. (1995) CD54 (ICAM-1)cluster report. In Leucocyte Typing V: White Cell Differentiation Antigens. S. Schlossman et al. eds.Oxford University Press pp.1548-1550. 2. -Ohlow Marc-Alexander,1 Michel Christoph,1 Ting–Hui Chen,1 Schmidt Andreas,2 Saenger Joerg,2 Lauer Bernward,1 and Brunelli MichelePredictors of long-term outcome in patients with biopsy proven inflammatory cardiomyopathy, Journal of Geriatric Cardiology, 2018, doi:10.11909/j.issn.1671-5411.2018.05.006

Safety Datasheet(s) for this product:NM_Thimerosal/wp-content/uploads/SDS/SDS Antibodies with Thimerosal Nordic-MUbio.pdf