| Clone | EP77 |
| Isotype | IgG |
| ProductType | PrimaryAntibodies |
| Units | 1ml |
| Host | Rabbit |
| Application | FlowCytometryImmunohistochemistry(frozen)Immunohistochemistry(paraffin) |
BackgroundTheantibodytoCD5isusedfortheclassificationoflymphoidneoplasm.CD5isexprimedbythemajorityofT-celllymphomaandisalsofoundonB-cellsinsomecasesofchroniclymphaticleukaemiaandcentrocyticlymphoma.Normallyitisnotfoundinotherformsoflymphoidneoplasme.g.follicularlymphoma,HairyCellLymphomaandLargeCellLymphoma.CD5isalymphocytesurfacemoleculewithamolecularweightof67kDa.Itisexprimedby95%ofthymocytesandapp.72%ofperipheralbloodlymphocytes.InlymphnodestheantigenisdetectedintheT-cellregionmainly.CD5isover-expressedinleukaemiaandT-celllymphomaandmayalsobedetectedinasubpopulationofactivatedB-cells.HumanCD5molecule.
Source
Immunogen:SyntheticProtein,correspondingtoasequenceofhumanCD5molecule
ProductAntibodysolutioninstABIlizingphosphatebufferpH7.3.Contains0.09%sodiumazide**.Thevolumeissufficientforatleast100immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002.
PurificationMethod:AntibodysolutioninstabilizingphosphatebufferpH7.3.Contains0.09%sodiumazide**.Thevolumeissufficientforatleast100immunohistochemicaltests(100µlworkingsolution/test).Useappropriateantibodydiluente.g.BIOLOGOArt.No.PU002.
SecondaryReagents:WerecommendtheuseofBIOLOGO"sUniversalStainingSystemDAB(Art.No.DA005)orAEC(Art.-No.AE005).
Specificity
SpeciesReactivity:Human
ApplicationsIHC(C,P),FACS
IncubationTime:60minatRT
WorkingConcentration:(liquidconc.)1:10-1:25
Pre-Treatment:Paraffinsectionsarepre-treatedwith1mMEDTA(pH8.0)inanautoclave(2minunderfullpressure,cooling15-20min.)
PositiveControl:Tonsil
Storage2-8°C
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.JonesN.H.,ClabbyM.L.,DialynasD.P.etal.(1986)IsolationofcomplementaryDNAclonesencodingthehumanlymphocyteglycoproteinT1/Leu-1.Nature323:346-349.2.KasaianM.T.,IkematsuH.andCasaliP.(1991)CD5+B-Lymphocytes(43250).ProceedingsoftheSocietyforExperimentalBiologyandMedicine197;226-241.3.ChanJ.K.C.,BanksP.M.,ClearyM.L.(1994)Aproposalforclassificationoflymphoidneoplasms(bytheinternationallymphomastudygroup).Histopathology25;517-536.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
