BackgroundTheantibodyiswellsuitedforthedetectionofCD4+cellsintissuesandcellsmears.UsuallyT-CelllymphomaintheskinareCD4+aswellasHTLV-1associatedLeukaemiaandlymphomarespectively.CloneMEM-115blocksbindingofHIVgp120toCD4+cellsandinhibitsalsoCD4-MHCClassIIinteractions.CD4isamembraneglycoproteinwithasinglechainofapp.54kDaMW.ItisexpressedbyasubgroupofT-Cells(Helper/Inducer-Cells)whichmakeapp.45%ofperipheralbloodlymphocytes.CD4isalsolocalizedonabout80%thymocytesandtoalowpercentageonmonocytes.Itisinvolvedinthedetectionofantigens,whicharepresentedbyantigenpresentingcells.CD4functionsasareceptorforHIV(HTLV).HumanCD4molecule,D1domain.

Source

Immunogen:PurifiedAntigenfromThymocyteandT-cellextract

ProductCellculturemediumwithphosphatebuffer,BSAand0.09%sodiumazide**.

PurificationMethod:Cellculturemediumwithphosphatebuffer,BSAand0.09%sodiumazide**.

SecondaryReagents:WerecommendtheuseofBIOLOGO"sUniversalStainingSystemDAB(Art.No.DA005)orAEC(Art.-No.AE005).

Specificity

SpeciesReactivity:Human

ApplicationsIHC(C,P)

IncubationTime:60minatRT

WorkingConcentration:(RTU)neat

Pre-Treatment:TheantigenepitopedetectedbycloneMEM-115maybesensitivetothetreatmentwithH2O2.Thereforeitisrecommendedtoblockendogenousperoxidaseswith0.5%H2O2inMethanol(10minincubation).Thisstepshouldbecarriedoutpriortoantigenunmasking!Antigenunmaskingisperformedat96-100°Celsiusin1mMEDTApH8.0for10-20min.

PositiveControl:Tonsil

Storage2-8°C

Caution*Theseantibodiesareintendedforinvitroresearchuseonly.Theymustnotbeusedforclinicaldiagnosticsandnotforinvivoexperimentsinhumansoranimals.**Thepreservativesodiumazideisknowntobepoisonousandpotentiallyhazardoustohealth.Itshouldbehandledonlybytrainedstaff.Despiteoftheproduct"slowazideconcentrationitmustbehandledwithcare.Disposeaccordingtoregionalrules!

References1.FaliniB,SteinH,PileriS,CaninoS,FarabbiR,MartelliMF,GrignaniF,FagioliM,MinelliO,CianiC,etal.Expressionoflymphoid-associatedantigensonHodgkin"sandReed-SternbergcellsofHodgkin"sdisease.Animmunocytochemicalstudyonlymphnodecytospinsusingmonoclonalantibodies.Histopathology.1987Dec;11(12):1229-42.2.SteinH,SpiegelH,HerbstH,NiedobitekG,FossHD.[LymphoidtissuesandAIDS:roleoflymphocytesandfolliculardendriticcells(FDC)]VerhDtschGesPathol.1991;75:4-19.Review.German.3.SpiegelH,HerbstH,NiedobitekG,FossHD,SteinH.Folliculardendriticcellsareamajorreservoirforhumanimmunodeficiencyvirustype1inlymphoidtissuesfacilitatinginfectionofCD4+T-helpercells.AmJPathol.1992Jan;140(1):15-22.4.DaumS,WeissD,HummelM,UllrichR,HeiseW,SteinH,RieckenEO,FossHD;IntestinalLymphomaStudyGroup.FrequencyofclonalintraepithelialTlymphocyteproliferationsinenteropathy-typeintestinalTcelllymphoma,coeliacdisease,andrefractorysprue.Gut.2001Dec;49(6):804-12.

ProteinReference(s)

DatabaseName:UniProt

Accessionnumber:P01730(CD4_HUMAN)

SpeciesAccession:Human