BackgroundThis antibody reacts extremely well with B cell lymphoma, in rare cases also T cell lymphoma may be stained. In Non-Hodgkin Lymphoma Reed Sternberg Cells will be stained. CD20 is a non-glycosylated membrane protein with a molecular mass of33 kDa, which is typical for resting B cells. B cell activation leads to higher molecular isoforms of the protein, which will also be detected by the antibody L26. The antigen is absent from plasma cells (CD138+). Human CD20cy on peripheral and lymphoidal B-cells, germ center of lymph knots..

Source

Immunogen: B-Cells from human Tonsils

ProductAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGOArt .No. PU002.

Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGOArt .No. PU002.

Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Specificity

Species Reactivity: Human

ApplicationsIHC(C, P), FACS

Incubation Time: 60 min at RT

Working Concentration: (liquid conc.) 1:10 to 1:50

Pre-Treatment: Improvement of staining with Unmasking Fluid G (Art. No. DE007) or Unmasking Fluid C (Art. No. DE000)

Positive Control: Lymph nodes, Tonsil

Storage2-8°C

Caution*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

References1. Zhou l.-Z. and Tedder T. (1993) CD20 Workshop panel report. In Schlossman S.F. et al. Eds. Leucocyte Typing V. White cell differentiation antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 511-514. 2. Takami T., Qi C.,Yamada T., et al. (1989) B20.3. Reactivity and Specificity of L26 (pan-B-cell mAb) on 322 cases of fresh and paraffin-embedded lymphoproliferative diseases. In Kapp W. et al. eds. Leucocyte Typing IV. White Cell Differentiation Antigens. Oxford University Press, Oxford, N.Y., Tokyo; pp 134-136. 3. Mason D.Y., Comans-Bitter WM., Cordell J.L., Verhoeven M.A.J. and van Dongen J.J.M. (1990) Antibody L26 recognizes an intracellular epitope of the B-cell-associated CD20 antigen. Am. J. Pathol. 136; 1215-1222.