| Clone | DEF-3 |
| Isotype | IgG1 |
| ProductType | PrimaryAntibodies |
| Units | 100µg |
| Host | Mouse |
| Application | FlowCytometryImmunohistochemistry(frozen)Immunohistochemistry(paraffin) |
BackgroundThemonoclonalantibodyDEF-3reactswithhumanalpha-defensins(HNP1-3),whicharesynthesizedandstoredbyneutrophilicgranulocytes.HumandefensinsareCys-richantimicrobialpeptideswhichspecificallyreactagainstGram-negativebacteria.Originallydefensinswereisolatedfromhemolysates,buttheycanalsobefoundonthecellsurfaceofepitheliaoftheurogenitaltract,lung,andtrachea.Inleukocytesdefensinsarestoredingranulesbeingreleasedintophagolysosomesforthedestructionofincorporatedmicroorganisms.Aminoacidsequencesofalpha-defensins1-4(HNP1-4)andbeta-Defensins1-4(HBD1-4)areknown.HumanNeutrophilPeptides(HNP1-3)syn.alpha-Defensins1-3,reactivitywithHNP-4nottested.
Source
Immunogen:NativeDefensins
ProductAffinitypurifiedIgG,lyophilizedfromPBS,BSA,andsodiumazide**,reconstitutewith0.5mlsterilewatertogetastocksolutionof200µg/ml.
PurificationMethod:AffinitypurifiedIgG,lyophilizedfromPBS,BSA,andsodiumazide**,reconstitutewith0.5mlsterilewatertogetastocksolutionof200µg/ml.
Concentration:200µg/mlIgGafterreconstitutionwith0,5mldistilledwater
SecondaryReagents:WerecommendtheuseofBIOLOGO"sUniversalStainingSystemDAB(Art.No.DA005)orAEC(Art.-No.AE005).
Specificity
SpeciesReactivity:Human,cross-reactivitywithrhesusmonkeyandprimatesexpected
ApplicationsIHC(C,P),FACS
WorkingConcentration:(lyophilisate)0.2µg/mlIHC(C),0.5µg/mlIHC(P)
Pre-Treatment:Formaldehyde-fixedtissuerequiresantigenunmaskingpreferredmethodsare0.1%Pronase,ProteinaseKorTrypsin.
PositiveControl:Infectedskinororalmucosa
Storage-20"°C"
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.
References1.GanzT.etal.(1985)J.Clin.Invest.,76;1427ff2.GanzT.,etal.(1992)Med.Microbiol.Immunol.181;99ff3.MizukawaN;SugiyamaK;FukunagaJ;UenoT;MishimaK;TakagiS;SugaharaT(1998)Defensin-1,apeptidedetectedinthesalivaoforalsquamouscellcarcinomapatients.AnticancerRes18(6B):4645-4649.4.LinzmeierR.,HoC.H.,HoangB.V.,andGanzT.(1999)A450kbcontigofdefensingenesonhumanchromosome8p23.Gene233;205-211.5.MikuzawaN.SugiyamaK.,KamioM.,YamachikaE.,UenoT.,FukunagaJ.,TakagiS.,SugaharaT.(2000)Immunohistochemicalstainingofhumanalpha-defensin-1(HNP-1)insuBMAndibularglandsofpatientswithoralcarcinomas.AnticancerRes.20(2B);1125-1127.
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
