| ProductType | PrimaryAntibodies |
| Units | 100µl |
| Host | Sheep |
| Application | ImmunohistochemistryWesternBlotting |
BackgroundThepolyclonalantibodyiswellsuitedforthedetectionof25(Hydroxy)VitaminD3-1-alpha-Hydroxylaseintissuesandtissueextractse.g.inHKC-8celllinefromhumanproximaltubules(1).Theseco-steroidhormone1,25-DihydroxyvitaminD3playsavitalroleinCalciummetabolism,intissuedifferentiationandduringthegrowthofbone.ThebiosynthesisofthehormoneiscatalysedbyamitochondrialP450-enzyme,25-Hydroxy-VitaminD31-alpha-Hydroxylase.Althoughtheenzymehasbeendetectedindifferenttissues,themainsiteofVitaminD3synthesisisthekidney.25(OH)VitaminD3-1-alpha-Hydroxylase,56kDaProteininWesternBlot.
Source
Immunogen:Syntheticpeptidefromthecodingsequenceofmurine25(OH)-Vitamin-D3-1-alpha-Hydroxylase(aa266-289)
ProductSheepIgGfractioninglycinebufferedNaClsolutionpH7.4;contains0.1%sodiumazide,0.1%Epsilon-amino-n-caproicacid,0.01%Benzamidineand1mMEDTA
PurificationMethod:SheepIgGfractioninglycinebufferedNaClsolutionpH7.4;contains0.1%sodiumazide,0.1%Epsilon-amino-n-caproicacid,0.01%Benzamidineand1mMEDTA
Concentration:app.7mg/ml
Specificity
SpeciesReactivity:Mouse,human
ApplicationsWB,IHC
WorkingConcentration:(liquidconc.)1/500(dependingonfixation,species,unmaskingmethodandsecondarysystem)
PositiveControl:Kidney
Storage-20°C
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.
References1.BlandR,WalkerEA,HughesSV,StewartPM,HewisonM.(1999)Constitutiveexpressionof25-hydroxyvitaminD3-1-alpha-hydroxylaseinatransformedhumanproximaltubulecellline:EvidencefordirectregulationofvitaminDmetabolismbycalcium.Endocrinology140(5),2027-2034.2.ZehnderD,BlandR,WalkerEA,BradwellAR,HowieAJ,HewisonM,StewartPM.(1999)Expressionof25-HydroxyvitaminD3-1-alpha-hydoxylaseinhumankidney.J.Am.Soc.Nephrol.10;2465-24733.BlandR,MarkovicD,HillsCE,HughesSV,ChanSL,SquiresPE,HewisonM(2004)Expressionof25-hydroxyvitaminD3-1alpha-hydroxylaseinpancreaticislets.JSteroidBiochemMolBiol.89-90(1-5):121-125.4.TakeyamaK,KitanakaS,SatoT,KoboriM,YanagisawaJ,KatoS.(1997)25-HydroxyvitaminD31alpha-hydroxylaseandvitaminDsynthesis.Science;277(5333):1827-30(cloningof1-alpha-Hydroxylase)
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
