BackgroundAccumulation of the amyloid-peptide (A ) in the cerebral cortex is a critical event in the pathogenesis of AlzheimerÕs disease. The amyloid protein precursor (APP) is cleaved by secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for secretase to generate the 4 kDa amyloid-peptide (A ), which is deposited in the brains of all suffers of AlzheimerÕs disease.

Synonyms: Rabbit anti-β-Amyloid Peptide Precursor (Amyloid-β Peptide N-terminal)

Source

Immunogen: Synthetic peptide corresponding to amino acids 653 to 662 of human amyloid A4 protein precursor (APP) or 1 to 10 of the 4kDa Amyloid-peptide generated by and-secretases.This sequence is identical to those of rabbit, pig, bovine, guinea pig and chicken.

Product

Product Form: Unconjugated

Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative

Purification Method: Antigen Immunoaffiinity Purification

Concentration: See vial for concentration

ApplicationsDetects APP and Ab peptide by Western blot at 0.5 to 1 µg/ml.Detects an approximately 120 kDa band in human, mouse and rat brain tissue lysates.Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Murine brain lysate

StorageProduct should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Shipping Conditions: Ship at ambient temperature, freeze upon arrival

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Ponte, P., et al.A new A4 amyloid mRNA contains a domain homologous to serine proteinase inhibitors. Nature 1988, 331, 525-527.2. Selkoe, D.J. Cell biology of the amyloid beta-protein precursor and the mechanism of Alzheimer's disease. Annu. Rev. Cell Biol. 1994, 10, 373-403

Protein Reference(s)

Database Name: SwissProt

Accession number: P05067

Species Accession: Human

Safety Datasheet(s) for this product:NM_Sodium Azide/wp-content/uploads/SDS/Antibody SDS with Sodium Azide Noridic-MUbio.pdf