Background Elastin is the major protein of elastic fibres that form a randomly orientated network in many tissues. Due to its high content of hydrophobic amino acids it is relatively resistant to chemicals and proteinase. Its principal function is to provide elasticity to tissues, however it has also chemotactic and cell adhesion properties. Human elastin, cross-reactivity in RIA at 1:500 dilution: human collagen type I, II, III, IV, V <0.1 %;="" human="" fibronectin="">< 0.1%.="">

Source

Immunogen: Purified elastin from human skin

ProductAffinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Purification Method: Prot A affinity purified antibody lyophilized from phosphate buffered solution; no BSA and preservative added!

Concentration: app. 1 mg/ml

Secondary Reagents: Anti-rabbit IgG-conjugates

SpecificityHuman elastin

Species Reactivity: Human, cross-reaction with rat < 0.1%="">

ApplicationsIFA, IHC(P), ELISA, RIA

Incubation Time: IHC(P) 60 min at RT or 2-8°C over night

Working Concentration: (purified, lyophilized) IFA ? 1:80, IHC(P) ? 1:500, ELISA ? 1:200 (OD ? 500)

Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended.

Positive Control: Human skin or aorta

Storage-20°C

CautionThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Nordic-MUbio​ accepts no liability for any inaccuracies or omissions in this information.

References1. Black A.F., Bouez C., Perrier E., Schlotmann K., Chapuis F., Damour O. (2005) Optimization and characterization of an Engineered human skin equivalent. Tissue Engineering 11, 723-733. 2. Guerret S., Govignon E., Hartmann D.J., Ronfard V. (2003) Long term remodeling of a bilayered living human skin equivalent (Apligraf®) grafted onto nude mice: immunolocalization of human cells and characterization of extracellular matrix. Wound Rep. Reg.3. Zsolt U., Peyrol S., Plauchu H., Zabot M.T., Lebwohl M., Schilling K., Green M., Boyd C.D., Csiszár K. (2000) Elastin gene deletions in Williams syndrome patients result in altered deposition of elastic fibers in skin and a subclinical dermal phenotype. P 4. Duplan-Perrat F., Damour O., Montrocher C., Peyrol S., Grenier G., Jacob M.P., Braye F. (2000) Keratinocytes influence the maturation and organization of the elastin network in a skin equivalent. J. Invest Dermatol 114, 365-370.

Protein Reference(s)

Database Name: UniProt

Accession number: P15502 (ELN_HUMAN)

Species Accession: Human

Elastin on human skin paraffin section. EL25011, DAB, hematoxylin