| Isotype | IgG1(F)/IgG2a(PE) |
| ProductType | Bi-TestªReagents(FITC/RPE) |
| Units | 100Tests |
| Host | Mouse |
| Speciesreactivity | Human |
| Application | FlowCytometry |
BackgroundIdentificationofCD4onhumanhelper/inducerTcellsexpressingthe60,000M.W.surfaceantigen(HLAclassIIreactive).CD4ispresentinlowdensityonmonocytes.Anti-humanCD28bindsthe44kDaMWcellsurfaceproteinonthesurfaceofmostTcells.TheCD26antigenrecognizestheenzymedipeptidylpeptidase(DPP),aserineprotease.ItsM.W.is120kDa.TheCD26antigenisassociatedwiththebindingoftheTATtransactivatingproteinofthehumanimmunodeficiencyvirus(HIV).
Synonyms:CD4FITC-CD26PE
Source
Immunogen:CD4=DerivedfromthehybridizationofmouseNS-1myelomacellswithspleencellsfromBALB/cmiceimmunizedwithhumanperherialbloodTlymphocytes.
Product
ProductForm:Bi-Test(FITC/RPE)Reagent
Formulation:Providedassolutioninphosphatebufferedsalinewith0.08%sodiumazideand0.2%carrierprotein
PurificationMethod:ProteinA/GChromatography
Concentration:Titeredforflowcytometry
ApplicationsPBMC:Add10µlofMAB/10^6PBMCin100µlPBS.Mixgentlyandincubatefor15minutesat2ºto8ºC.WashtwicewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.WHOLEBLOOD:Add10µlofMAB/100µlofwholeblood.Mixgentlyandincubatefor15minutesatroomtemperature20ºC.Lysethewholeblood.WashoncewithPBSandanalyzeorfixwith0.5%v/vofparaformaldehydeinPBSandanalyze.Seeinstrumentmanufacturer’sinstructionsforLysedWholeBloodandImmunofluorescenceanalysiswithaflowcytometerormicroscope.
FunctionalAnalysis:FlowCytometryStaining
StorageProductshouldbestoredat4-8ºC.DONOTFREEZE
ProductStABIlity:Reagentsarestablefortheperiodshownontheviallabelwhenstoredproperly
ShippingConditions:RoomTemperature
CautionThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Itmaycontainhazardousingredients. PleaserefertotheSafetyDataSheets(SDS)foradditionalinformationandproperhandlingprocedures.Disposeproductremaindersaccordingtolocalregulations.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.
References1.ThymusDependentMembraneAntigensinMan:InhibitionofCell-MediatedLympholysisbyMonoclonalAntibodiestotheTH-2Antigen.Evans,R.L.,Wall,D.W.,Platsoucas,C.D.,Siegal,F.P.,Fikrig,S.M.,Testa,C.M,andGood,R.A.Proc.Nat.Acad.Sci.78,544,1981.2.NovelImmunoregulatoryFunctionsofPhenotypicallyDistinctSubpopulationsofCD4+cellsintheHumanNeonate.Clement,L.T.,Vink,P.E.,Bradley,G.E.J.Immunology145(1):102-8,1990.3.AntigenPresentationbytheCD4PositiveMonocyteSubset.Szabo,G.,Miller,C.L.,Kodys,K.,J.Leukoc.Biol.47(2):111-20,1990.4.HumanImmunodeficiencyVirusInfectionisEfficientlyMediatedbyaGlycolipid-AnchoredformofCD4.Diamond,D.C.,Finberg,R.,Chaudhuri,S.,Sleckman,B.P.,Burakoff,S.J.,Proc.Natl.Acad.Sci.87(13):5001-5,1990.5.DevelopmentRegulationoftheIntrathymicTcellPrecursorPopulation.Adkins,B.,J.Immunol.146(5):1387-93,1991.6.InductionofCD4andSusceptibilitytoHIV-1InfectioninHumanCD8+TLymphocytesbyHumanHerpesvirus6.Lusso,P.,DeMaria,A.,Malnati,M.,Lori,F.,DeRocco,S.E.,Baseler,M.,Gallo,R.C.,Nat.349(6309):533-5,1991.7.CD26antigenisasurfacedipeptidylpeptidaseIV(DPPIV)ascharacterizedbymonoclonalantibodiescloneTII-19-4-7and4EL1C7.ScandJImmunol1990Ap;31(4):429-35UlmerAJ;MatternT;FellerAC;HeymannE;FladHD8.Comitogeniceffectofsolid-phaseimmobilizedanti-1F7onhumanCD4TcellactivationviaCD3andCD2pathways.SO-JImmunol1990Jun;144(11):4092-100AU-DangNH;TorimotoY;DeuschK;SchlossmanSF;MorimotoC9.Comitogeniceffectofsolid-phaseimmobilizedanti-1F7onhumanCD4TcellactivationviaCD3andCD2pathways.JImmunol1990Jun;144(11):4092-100DangNH;TorimotoY;DeuschK;SchlossmanSF;MorimotoC10.BindingoftheTcellactivationmonoclonalantibodyTa1todipeptidylpeptidaseIV.JLeukocBiol1990Oc;48(4):291-6BartonRW;PrendergastJ;KennedyCA11.ExpressionofCD26(dipeptidylpeptidaseIV)onrestingandactivatedhumanT-lymphocytes.ScandJImmunol1991Ju;33(6):737-48MatternT;ScholzW;FellerAC;FladHD;UlmerAJ12.Inductionofinterleukin2(IL2)andinterferon-gammaandenhancementofIL2receptorexpressionbyaCD26monoclonalantibody.EurJImmunol1991Ap;21(4):1085-8PlanaM;VinasO;DelaCalle-MartinO;LozanoF;Ingles-EsteveJ;RomeroM;Alberola-IlaJ;YagueJ;VilellaR;VivesJ13.Lymphocyteimmunophenotypesamonganti-HTLV-I/II-positiveblooddonorsandrecipients.TheTransfusionSafetyStudyGroup.JAcquirImmuneDeficSyndr1991;4(6):628-32FletcherMA;GjersetGF;HassettJ;DoneganE;ParkerJW;MosleyJW
ProteinReference(s)
DatabaseName:UniProt
Accessionnumber:P01730,P27487
SafetyDatasheet(s)forthisproduct:NM_SodiumAzide/wp-content/uploads/SDS/AntibodySDSwithSodiumAzideNoridic-MUbio.pdf
