Background:

HumanGFAP-δ,alsotermedGFAP-ε,isanalternativesplicevariantoftheGFAPprotein.GFAPproteinsareselectivelylocatedinastrocytesandrepresentthemajorconstituentofastrocyticintermediatefilaments.GFAPexpressionlevelsarehighlyvariableduringdevelopmentofthecentralnervoussystem.GFAP-δdiffersfromthepredominantspliceformofGFAP,GFAP-α,initsC-terminalsequenceinwhichtheoriginalaminoacidsequence,encodedbyexons8and9,arereplacedbyauniqueandconservedtailof42aminoacidsencodedbyexon7+andcalledtheGFAP-δtaildomain.ThisGFAP-δtaildomainisabletobindpresenilinproteinsofwhichmutatedformsareacauseofAlzheimerdisease.GFAP-δdoeshowevernotdiscriminatebetweenAlzheimerandcontrolfissue.UpregulationofGFAP-δhasanegativeeffectonGFAPfilamentformation.IncontrasttoGFAP-α,GFAP-δisnotupregulatedinastrogliosis.

Source:

GFAP-δisarabbitpolyclonalantiserumderivedbyimmunizationwiththepeptideQAHQIVNGTPPARG,representingthelast14C-terminalaminoacidsoftheGFAP-δprotein.

Product:

Eachvialcontains100μlrabbitpolyclonalserumcontaining0.09%sodiumazide.

Applications:

GFAP-δissuitableforimmunohistochemistryonparaffinsectionsandWesternblotting.Optimalantibodydilutionshouldbedeterminedbytitration;recommendedrangeis1:100—1:500forimmunohistochemistryand1:50—1:1000forWesternblotting.

Specificity:

GFAP-δstainstheastrocyteslocatedinthesubpialzoneofthecerebralcortex,thesubventricularzoneofthehippocampusand,mostintensely,aribbonofastrocytesfollowingtheependymallayerofthecerebralventricles.

Storage:

Storeat4°C,orinsmallaliquotsat-20°C.

References:

1.Roelofs,R.F.,Fischer,D.F.,Houtman,S.H.,Sluijs,J.A,vanHaren,W.,vanLeeuwen,F.
W.,andlVbl,E.M.(2005).Adulthumansubventricular,subgranular,andsubpialzones
containastrocyteswithaspecializedintermediatefilamentCytoskeleton,GLIA52,289-300.
2.NielsenAL.,Holm,l.E.,Johansen,M,Bonven,B.,Jorgensen,P.,Jorgensen,A.L.,
(2002).Anewsplicevariantofglialfibrillaryacidicprotein,GFAPepsilon,interactswiththe
presenilinproteins.J.Biol.Chem.277,29983-91.

Caution:

ThisproductisintendedFORRESEARCHUSEONLYandFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisproductcontainssodiumazide.Topreventformationoftoxicvapors,donotmixwithstrongacidicsolutions.Topreventformationofpotentiallyexplosivemetallicazidesinmetalplumbing,alwayswashintodrainwithcopiousquantitiesofwater.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioBVacceptsnoIiABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。