Background:

Pirimiphosisaninsecticidebelongingtothegroupoforganophosphothioates.Itisappliedinthefieldonfruits,vegetables,inthestorageofgrainproducts,andasanadditiveinpaints.IthasadverseeffectsonalgaeandmammalsduetoitsBIOLOGicalactivityasacetylcholineesteraseinhibitor.

Source:

Rabbitimmuneserum

Product:

Eachvialcontains0.1mlrabbitpolyclonalantiserum.

Applications:

TheantibodymaybeusedinELISAandImmuno-affinity-chromatography(IAC).Suggesteddilution1:100,000inELISA(thistiterisdefinedasthedilutionthatgives50%oftheabsorbancefromthemaximumabsorbancewhentestedwithELISA).Platesarecoatedwith400ng/mlovalbumine-conjugatedpirimiphos.HRP-conjugatedanti-rabbitIgGshouldbeusedasatracerina1:8,000dilution.

CrossReactivity:

Thespecificityoftheantibodywasdeterminedbymeasuringthecross-reactivityinELISAforarangeofcompounds.CrossReactivityCompoundtested%CrossReactivityPirimiphos100%(2,4-dichlorophenoxy)aceticacid<0.1% Chloropiriphos <0.1% Deltametrin <0.1% Fenitrothion <0.1% Permethrin <0.1% Etrimphos <0.1% Atrazine <0.1% Methacrimphos <0.1% Aldicarb <0.1% Malathion <0.1% Tebuconazole <0.1%

Specificity:Immunogen:BSA-Pirimiphos-methylConjugate,Target:Pirimiphos-methyl,CASno.:29232-93-7,Solubility:Water,acetone,ethanol,propanol,toluene

Storage:

Storeat4°Cor-20°C.Theworkingantibodysolutionisstableforatleast7daysat4°C.Precautionsshouldbetakenforstorageforlongerperiods.ProblemsoflongtermstABIlitymayoccurwithhighlydilutedsolutions.Nootherpreservativeagenthasbeenaddedtothepresentformulation.Forlongstoragepurposesinsolutiontheadditionofsodiumazideto0.02%isadvisedwiththeappropriateprecautionsofuse.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisproductcontainssodiumazide.Topreventformationoftoxicvapors,donotmixwithstrongacidicsolutions.Topreventformationofpotentiallyexplosivemetallicazidesinmetalplumbing,alwayswashintodrainwithcopiousquantitiesofwater.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。