Background:

Alkylphenolsareusedasstartingmaterialfortheproductionofsurfaceactivesubstances,detergents,pesticides,paints,textileandpaper.Itisreleasedduringproductionanduseoftheseproducts.Alkylphenolsarerecognizedasendocrinedisruptors,inparticularestrogenicendocrinedisruptors.Ahighlycomparablecompoundisoctylphenol.

Source:

Rabbitimmuneserum

Product:

Eachvialcontains0.1mlofrabbitpolyclonalantiseruminphosphatebufferedsaline(PBS;pH7.2)containing0.02%sodiumazide.

Applications:

TheantibodymaybeusedinELISAandImmuno-affinity-chromatography(IAC).Thetiteroftheantibodyisapproximately100,000(thetiterisdefinedasthedilutiongiving50%binding).

CrossReactivity:

Theantibodywastestedforspecificityusing2differentconjugates:BSA-C6-APandBSA-C8-AP.CrossReactivityBSA-C6-APBSA-C8-AP4-Octylfenol100%100%4-tert-Butylphenol67%27%2-sec-Butylphenol8%1%4-pentylphenol1167%2000%4-n-heptylphenol142%133%4-n-propylphenol500%250%2-n-propylfenol0,20%0%4-Isopropylphenol333%100%4-n-hexylphenol667%1667%4-chloro-2-cyclo-hexylphenol0%1%nonylphenol(tech)20%134%4-n-nonylphenol42%40%bisphenolA4%7%4-cumylphenoln.d.10%4,4"-(ethylidene)bisphenoln.d.10/%Bisphenol-An.d.0,1%BisphenolAdiglycidylethern.d.1%4,4"cyclohexylidenebisphenoln.d.0,1%Bis-(4-hydroxyphenyl)-methanen.d.1%

Specificity:

Immunogen:BSA-C8-alkylphenolconjugate,Target:Alkylphenols(afamilyofcomparablecompounds;asanexamplenonylphenolisgiven),CASno.:104-40-5,Solubilitytarget:alcohols

Storage:

Thisantibodyworkingsolutionisstableforatleast7daysat4°C.Precautionsofstorageshouldbetakenforlongerperiods.ProblemsoflongtermstABIlitymayoccurwithhighlydilutedsolutions.Nootherpreservativeagenthasbeenaddedtothepresentformulation.Forlongstoragepurposesinsolutiontheadditionofsodiumazideto0.02%isadvisedwiththeappropriateprecautionsofuse.

References:

1.MeulenbergEP,KoopalK,RhemrevR.(2005)Immunoassaysforalkylphenolicpollutantswithendocrinedisruptingactivity.Intern.J.Environ.Anal.Chem.85,871-883.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisproductcontainssodiumazide.Topreventformationoftoxicvapors,donotmixwithstrongacidicsolutions.Topreventformationofpotentiallyexplosivemetallicazidesinmetalplumbing,alwayswashintodrainwithcopiousquantitiesofwater.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-Mubioacceptsnoliabilityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。