Background:

Thisstandardispreparedbypoolingserumobtainedfromalargenumbermicebelongingtoa-allotypeandb-allotypestainswiththefollowingassignedvalues:IgG1--2.00mg/ml.IgG2a--8.54mg/ml.IgG2b--1.25mg/ml.IgG3--0.754mg/ml.IgA--2.75mg/m.IgM--0.277mg/ml.ThedeterminationoftheconcentrationofeachofthefoursubclasseswascarriedoutbysinglerADIalimmunodiffusion(RID,Mancini)inagargelNordicNr.2usingsubclass-specificpolyclonalantiseraandpurifiedmouseimmunoglobulinsasprimarystandards.AllotypicdifferencesinmouseIgG2amayinterferewiththedeterminationofthissubclass.AtleasteightallelicvariantsofIgG2ahavebeenidentified.Mostlaboratorymousestrainsfallintooneoftheseallogeneicgroups.MouseStandardSerumNOR-02containsIgG2aofbothallelictypesIgh-1aandIgh1bandcabbeusedforthequantitativedeterminationofIgG2ainthemajorityofmousestrains,includingBalb/C,C3H,CBA/J,SJL/JandSM/J.MousestandardserumNOR-02isintendedforuseasacalibratortopreparestandardcurvesinquantitativedeterminationsoftheimmunoglobulinsIgAandIgMandIgGsubclasses.

Product:

Delipidated,lyophilizedserum.Nopreservativeadded

Applications:

Theproductisshippedlyophilizedatambienttemperature.Thelyophilizedcontentisreconstitutedbyadding0.5mlsterilewater,mixedgentlybyinversionuntilcompletedissolutionisobtained.Allowtostandatambienttemperaturefor5-10minutestoreachequilibrium.Reconstitutedserummaybedividedintosmallaliquots,frozenatorbelow-20°C.

Specificity:

MousestandardSerumwithassignedvaluesforIgA,IgMandIgGsubclasses.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。