Background:

ThereactivityoftheantiserumisdirectedtotheFcsubunitoftheIgAmoleculewhichexpressesstrictisotypic(class)specificity.Itdoesnotreactwithanynon-Igproteinindogserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.Inenzyme-immunocytochemicalandimmunohistochemicalstainingforthedetectionofIgAatthecellularandsubcellularlevelbystainingofappropriatelytreatedcellandtissuesubstrates;todemonstratecirculatingIgAantibodiesinSEROdiagnosticmicroBIOLOGyandautoimmunediseases;toidentifyaspecificantigenusingareferenceantibodyofdogoriginknowntobeoftheIgAisotypeinthemiddlelayeroftheindirecttestprocedure;innon-isotopicassaymethodology(e.g.ELISA)tomeasureIgAindogserumorotherbodyfluids.AntiseratoIgAdonotdiscriminatebetweenserumIgA(monomericanddimeric)andhighermolecularformssuchassecretoryIgA.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

PurifiednormalIgAisolatedfrompooleddogserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Horseradishperoxidase-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

Specificity:

Horseradishperoxidase-conjugatedIgGfractionofpolyclonalGoatantiSerumtoDogIgA,Fcspecific

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

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