Background:

Testedinimmunoelectrophoresis,usingdifferentantigen/antibodyconcentrationratio’stheantiserumgivesonecharacteristicprecipitinline.Inenzyme-immunocytochemicalandimmunohistochemicalstainingofconcanavalinAinappropriatelypreparedsubstrates.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.Theentiretestsystemmustbefreeofsodiumazide,sinceitinhibitstheenzymeactivity.

Source:

ThelectinconcanavalinAisacell-agglutinatingproteinreactingspecificallywithmoleculeswhichcontainalpha-D-mannopyranosyl,alpha-D-glucopyranosylandstericallyrelatedresidues.AtpH4.5-5.6itexistasadimer,abovepH7.0predominantlyasatetramer.Themonomerhasamolecularweightofabout52,000.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

HorserADIshperoxidase-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(paraffin),Dotblot,Immunoblotting.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiessincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.Thedegreeofcross-reactivityisalsodependentontheconcentRationofthereactantsandthesensitivityoftheassayarrangement.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

Specificity:

Horseradishperoxidase-conjugatedIgGfractionofpolyclonalGoatantiSerumtoconcanavalinAfromjackbean

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyinthedarkatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

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