Background:

ThereactivityoftheantiserumisdirectedtotheFcandFabsubunitsoftheIgGmolecule.ItincludessomereactivitywithotherimmunoglobulinsviathecommonFabportion.Itdoesnotreactwithanynon-Igproteininbovineserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.ToidentifyandmeasureIgG,antigenorantibody,atthecellularandsubcellularlevelbyimmunofluorescencestainingofappropriatelytreatedcellandtissuesubstrates,andtodemonstratecirculatingantibodiesinSEROdiagnosticmicroBIOLOGyandautoimmunediseases;toidentifyaspecificantigenorimmunecomplexusingareferenceantibodyofbovineorigininthemiddlelayeroftheindirecttestprocedure.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

PurifiednormalIgGisolatedfrompooledbovineserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

FITC-coupledpurifiedhyperimmunegoatIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded.Itisreconstitutedbyadding2mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail,howevertheconjugatehasbeentestedindoubleradialimmunodiffusion(Ouchterlony)antiHumanandSwineSerumandnoreactionhasbeenobserved.

Specificity:

Fluoresceinisothiocyanate-conjugatedIgGfractionofpolyclonalGoatantiSerumtoBovineIgG,heavyandlightchains

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyinthedarkatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

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