Background:

ThereactivityoftheantiserumisdirectedtotheFcandFabsubunitsofbothsubclasses,IgG1andIgG2.ItincludesacertaindegreeofreactivitywithotherimmunoglobulinsviathecommonFabportion.Inimmunoelectrophoresisagainstgoatserumasinglecharacteristicprecipitinlinewithaspurisusuallyobtained,representingprimarilyIgG2.IftheIgG1levelinthesampleapproachestheconcentrationofIgG2,itmaybecomevisIBLeasasecondprecipitinline.Itdoesnotreactwithanynon-Igproteiningoatserum,astestedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion.Asunlabelledprimaryorsecondaryreagentforindirectdetectiontechniques,toprepareconjugateswithMarkersoftheuser’sownchoice,toprepareaninsolubleimmunoaffinityadsorbentorasolidphaseantibodyreagentbycouplingtoanartificialcarrierandascatchingordetectionantibodyinnonisotopicmethodologyandsolidphaseimmunochemistry.Whenappliedinanycytochemicalorhistochemicalprocedureorsolidsphasecouplingtechnique,theoptimumconcentrationoftheIgGpreparationshouldalwaysbeestablishedbytitration.

Source:

PurifiednormalIgGisolatedfrompooledgoatserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

PurifiedhyperimmunedonkeyIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2).Nopreservativeadded,asitmayinterferewiththeantibodyactivity.Noforeignproteinadded.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

Indirectimmunofluorescence,ELISA,Dotblot,Immunoblotting.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoimmunoglobulins,sinceIgofdifferentspeciesfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiserumhasbeentestedindoubleradialimmunodiffusionwiththefollowingresults:bovine++guineapig±mouse±rat±cat±hamster-monkey+sheep++chicken-horse-pigeon-swine-dog±human+rabbit±turkey-Anegativecross-reactionindoubleradialimmunodiffusiondoesnotexcludesomereactioninmoresensitivetechniques.

Specificity:

PurifiedIgGfractionofpolyclonalDonkeyantiSerumtoGoatIgG,heavyandlightchains

Storage:

ThelyophilizedIgGfractionisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheproduct

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。