Background:

Greenfluorescentprotein(GFP)isa27kDproteinwhichwasoriginallyidentifiedinthephotoorgansofAequoreavictoria(A.victoria)jellyfish.GFPisanaturallyfluorescentproteinwhichemitsgreenlightatamaximumwavelengthof509nmwhenexcitedbyblueorUVlight.InA.victoria,calciumionsbindandactivatetheproteinaequorincausingthereleaseofbluefluorescence,whichisthenabsorbedbyGFPresultinginthereleaseofgreenfluorescence.Inthelaboratory,theGFPproteinhasbeenusedextensivelyasareportermoleculetolabel,andstudy,cellularandsubcellularproteinsinlivingcellsusingawiderangeofBIOLOGicalapplications,includingoncology,cardiovasculardiseases,brainresearch,embryologyandplantsciences,justtonameafew.Forthequantitationoftheexpressionofaspecificprotein,taggedwithGFPinthesemodelsystems,antibodiestoGFPhaveproventobeofvalueinimmunoblottingstudiesandELISAprotocols.

Source:

WildtyperecombinantGFP(fromA.victoria)expressedinE.coliandpurifiedbyhighresolutiongelfiltration.

Product:

Eachvialcontains100µg1mg/mlpurifiedrecombinantGFP(wildtype)in10mMTRISbufferpH8.0,containing0.02%sodiumazide.

Specificity:

ThepurityoftherecombinantGFP(wildtype)proteinis>98%,confirmedbySDS-polyacrylamidegelelectrophoresis,specificabsorbancepeaks,HPLCandA397/280ratios.

Storage:

Maintainat-20°Cinundilutedaliquotsforupto24monthsafterdateofreceipt.Avoidrepeatedfreeze-thawcycles.

References:

ChalfieM,TuY,EuskirchenG,WardWW,PrasherDC.(1994)GreenfluorescentproteinasaMarkerforgeneexpression.Science263:802-805.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

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