Background:

Inimmunoelectrophoresisagainstpooledserumandenrichedserumproteinsfractionsprecipitationcanbeobservedofnotlessthan20individualproteinscomponents.InprecipitatingtechniquesasimmunoelectrophoresisandrADIalimmunodiffusion(Ouchterlony)toidentifytheserumproteinpattern,orthepresenceorabsenceofanindividualcomponent.Toevaluatethepurityofanisolatedserumproteinincludingimmunoglobulins.Sinceimmunoprecipitationdependsonacorrectantigen/antibodyconcentrationratio(zoneofequivalence)inthegelmedium,theproteinanalysisbyimmunoelectrophoresisofserumoranyotherBIOLOGicalfluidorproteinfractionshouldincludedifferentproportionsofthereactants.ItisnotpossIBLetoobtainanoptimalproteinpatterninasingleanalysis.Theelectroendosmosiseffectofdifferenttypesofagaronproteinswithadifferentnetchargecanbeusedtooptimizetheresolutionpowerofthetestsystem.AgarNordicNr.2containssufficientpositivelychargedionstooptimizetheresolutionoftheproteinsinthebeta-gammaregions,whilethealpharegionswillbecomemoredense.Highlypurifiedagar(AgarNordicnr.1)withlowelectroendosmosisfavourstheresolutionoftheproteinsinthealpharegions,whilethemajorcomponentsinthebeta-gammaregioncanstillbeidentified.

Source:

Pooledwholedogserumandpartlypurifiedserumfractions.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Delipidated,heatinactivated,lyophilized,stablewholeantiserum.Nopreservativeadded.TotalproteinandIgGconcentrationsintheantiserumarecomparabletothoseofpoolednormalgoatserum.Noforeignproteinsadded.Reconstitutethelyophilizedantiserumbyadding1mlsteriledistilledwater.

Applications:

Precipitationassays.Inimmunoelectrophoresisuse2μlserumorequivalentagainst120μlantiserum.Indoubleradialimmunodiffusion(Ouchterlony)usearosettearrangementwith10μlantiserumin3mmdiametercenterwelland2μlserumsamples(neatandseriallydilutedin2mmdiameterperipheralwells.Differentbleedingsoftheimmunizedanimalsarepooledtoobtainabroadspectrumbalancedagainstthevaryingconcentrationsoftheindividualserumproteincomponents.

CrossReactivity:

Inter-speciescross-reactivityisanormalfeatureofantibodiestoanimalproteinssincehomologousproteinsofdifferentspeciesfrequentlyshareantigenicdeterminants.ThisantiSerumhasnotbeenadsorbedforsuchcross-reactivity.Consequentlyitisnotspecies-specific.

Specificity:

PrecipitatingpolyclonalGoatantiSerumtoDogSerumproteins

Storage:

Thelyophilizedantiserumisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Dilutionsmaybepreparedbyaddingphosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheantiserum.Dilutedantiserumshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。