Background:

CD15isacarbohydratethatisreferredtoastheLewisXantigen,alsoknownasalpha-(1,3)-fucosyltransferase4,whichisexpressedonavarietyofglycolipidsandglycoproteinsfoundonthecellsurfaceofneutrophils,monocytes,eosinophilsandnon-hematopoietictissuesincludingembryonictissues.CD15isreportedtofunctionasanadhesionmoleculeandisinvolvedinchemotaxisandphagocytosis.ExpressionofCD15isalsoassociatedwithawidevarietyoftumourcellsincludingsomeadenocarcinomasandcertaintypesofmyeloidandlymphoidleukaemias.Reed-Sternbergcells,foundincasesofHodgkinsLymphoma,alsoexpressCD15andantibodiesrecognisingtheCD15antigenareoftenusedtohelpdifferentiatebetweenHodgkinsandnon-Hodgkinslymphoma.

Source:

BRA4F1isamouseIgMmonoclonalantibodyderivedfromthefusionofSP2.0-Ag14mousemyelomacellswithsplenocytesfromaBALB/cmouseimmunizedwithK562cells.

Product:

Eachvialcontains100µl1mg/mlpurifiedmonoclonalantibodyinPBScontaining0.09%sodiumazide.Theproductisdeliveredinlyophilisedformat.

Applications:

TheBRA4F1antibodyissuitableforthedetectionofCD15byflowcytometry,immunocytochemistyandimmunohistochemistryoffrozenandparaffinembeddedtissues.Optimalantibodydilutionsforthedifferentapplicationsshouldbedeterminedbytitration.

Specificity:

TheantibodyBRA4F1isdirectedagainsthumanCD15.TheantibodydoesnotcrossreactwiththesialylatedformofCD15(CD15s).

Storage:

Storeat4oC,orinsmallaliquotsat-20oC.

References:

1.ChorváthB,SedlákJ,SulikováM,PleskováI,AugustínováM,DurajJ.Monoclonalantibodies(seriesBra-)ofbroad(non-lineage)orrestricted(myelomonocytic)reactivitieselicitedwithgranulocytesorK562cells.Neoplasma.1989;36(6):633-42.2.CD

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisproductcontainssodiumazide.Topreventformationoftoxicvapors,donotmixwithstrongacidicsolutions.Topreventformationofpotentiallyexplosivemetallicazidesinmetalplumbing,alwayswashintodrainwithcopiousquantitiesofwater.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。