Background:

Indole-3-butyricacid(IBA)isaplanthormonebelongingtothegroupofauxins.Itisusedtostimulaterootformationinplantcuttings.AccordingtonewECregulations,IBAlevelsshouldapproachzeroatthetimetheplantletsareenteringthemarket.InordertobeabletomeasureIBAinsoilsamples,polyclonalantibodiesandacorrespondingELISAwasdeveloped.ThehighlypurifiedBSA-Indole-3-butyricacidconjugatecanbeusedasastandardinantibody-basedtests.

Product:

Eachvialcontains100µg1mg/mlBSA-Indole-3-butyricacidconjugateinPBS,pH7.4.
TheconjugatewaspurifiedandisolatedusingaBioradEcono-Pac10DGcolumnwithHSBasmobilephaseaccordingtotherecommendationsofthemanufacturer.

Applications:

TheBSA-Indole-3-butyricacidconjugateissuitableasastandardforuseinWesternblottingandELISA.Optimalproteindilutionsforthedifferentapplicationsshouldbedeterminedbytitration.

Storage:

Maintainat-20°Cinundilutedaliquotsforupto24monthsafterdateofreceipt.Avoidrepeatedfreeze-thawcycles.

References:

MeulenbergEP(2015)ThedevelopmentandapplicationofELISAfor3-Indole-butyricacid.ImmunochemImmunopathol:OpenAccess1:1000104.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

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