Background:

ConfirmedbyimmunoelectrophoresisanddoublerADIalimmunodiffusion(Ouchterlony).Notlessthan98%asdeterminedbyagar-gelelectrophoresis,quantitativedensitometryimmunoelectrophoresisanddoubleradialimmunodiffusion(Ouchterlony).PurifiedhumanIgG14kappamaybeusedasareferenceantigen,calibrator,coatingproteinandblockingagentinavarietyofimmunoassaysincludingimmunodiffusion,immunoelectrophoresis,ELISA,Westernblotting,dot-immunobindingassays(DIBA),haemagglutination,andcell-bindingassays.

Product:

Lyophilized,stablewhitepowder.NopreservativeaddedThelyophilizedcontentisreconstitutedbyadding0.5mlofsteriledistilledwater,mixedgentlybyinversionuntilcompletedissolutionisobtained.

Specificity:

ChromatographicallypurifiedHumanmyelomaIgG4kappa,allotypeGm4a.

Storage:

Thisproductisshippedlyophilizedatambienttemperature.Afterarrivalstoreat+4°C.Prolongedstoragemaybeat-20°C.Allowtostandatambienttemperaturefor5-10minutestoreachequilibrium.Reconstitutedsolutionmaybestoredfrozen.Workingdilutionsmaybepreparedbyaddingtherequiredamountofphosphatebufferedsaline(PBS),shouldnotberefrozenandpreferablyusedwithin24hours.Repeatedthawingandfreezingshouldbeavoided.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugationandwillnotaffecttheperformanceoftheproduct.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。