Background:

Inimmunoelectrophoresisagainstfreshdogserum,asingleprecipitinlineisobtainedinthebeta-1regionrepresentingnativeC3.AgainstserumcontainingpartlyactivatedC3,aprecipitinlineisobtainedwhichextendsfromthebeta-1intothealpha-2region,demonstratingagrADIent.InoldserumcontainingtotallyactivatedC3asingleprecipitinlineinthealpha-2regionisobtained.AntiseratoC3ccabalsoreactwiththefragmentsC3b,C3biandsmallerfragments,sincetheyallcarryantigenicdeterminantsoftheC3cdomain.Theproductdoesnotreactwithanyotherproteinscomponentofdogserumorplasma.ThefluorescentimmunoconjugatetodogC3cisusedtodeterminethepresenceandpatternofC3intissuelesionsusingimmunohistochemicalstainingtechniques.Locallydepositedimmunecomplexesintissueusuallycontaincomplement,pointingtoactivationoftheclassicalpathway.Complementactivationinvivoimpliesactivediseaseandmaycontributetotheelicitationofthepathogenesisandheextentoftissuedestruction.Sometimesthediagnosiscanbebasedondirectlyonlaboratoryfindings.Thisimmunoconjugateisnotpre-diluted.Theoptimumworkingdilutionofeachconjugateshouldbeestablishedbytitrationbeforebeingused.Excesslabelledantibodymustbeavoidedbecauseitmaycausehighunspecificbackgroundstainingandinterferewiththespecificsignal.

Source:

C3cisthemajorfragmentresultingfromC3cleavagebyC3convertaseandfactorI.Itiscomposedofanintactbetachainboundtotwofragmentsofthealphachain.C3cisisolatedandpurifiedfrompoolednormaldogserum.Freund’scompleteadjuvantisusedinthefirststepoftheimmunizationprocedure.

Product:

Fluorochrome-coupledpurifiedhyperimmuneIgGlyophilizedfromasolutioninphosphatebufferedsaline(PBS,pH7.2)Nopreservativeadded,asitmayinterferewiththeantibodyactivity.Itisreconstitutedbyadding1mlsteriledistilledwater,spundowntoremoveinsolubleparticles,dividedintosmallaliquots,frozenandstoredatorbelow-20°C.

Applications:

ELISA,Immunocytochemistry,Immunohistochemistry(frozen),(In)directimmunofluorescence.

CrossReactivity:

TheantiSerumdoesnotcross-reactwithanyothercomponentofDogplasma.Inter-speciescrossreactivityisanormalfeatureofantibodiestoplasmaproteinssincetheyfrequentlyshareantigenicdeterminants.Cross-reactivityofthisantiSerumhasnotbeentestedindetail.

Specificity:

Fluoresceinisothiocyanate-conjugatedIgGfractionofpolyclonalGoatantiSerumtoC3cfragmentofDogcomplementfactorC3

Storage:

Thelyophilizedconjugateisshippedatambienttemperatureandmaybestoredat+4°C;prolongedstorageatorbelow-20°C.Priortouse,analiquotisthawedslowlyinthedarkatambienttemperature,spundownagainandusedtoprepareworkingdilutionsbyaddingsterilephosphatebufferedsaline(PBS,pH7.2).Repeatedthawingandfreezingshouldbeavoided.Workingdilutionsshouldbestoredat+4°C,notrefrozen,andpreferablyusedthesameday.Ifaslightprecipitationoccursuponstorage,thisshouldberemovedbycentrifugation.Itwillnotaffecttheperformanceoftheimmunoconjugate.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

Noridic-Mubio是荷兰的一家著名的免疫学试剂生产厂商，因收购An Der Grub Bio Research GmbH(ADG)，增加了自己的产品线：全球最好的固定破膜剂FIX&PERM 。Nordic-Mubio严格遵照ISO9001标准生产高质量的抗体，主要为针对细胞粘附蛋白、核蛋白和骨架蛋白的抗体。同时Nordic-Mubio也是斑马鱼抗体开发的引领者之一，拥有50多种可用于斑马鱼免疫荧光检测的抗体。