Background:

CD27(ClusterofDifferentiation247)isatumornecrosisfactorreceptor.Atumornecrosisfactorreceptor(TNFR),ordeathreceptor,isatrimericcytokinereceptorthatbindstumornecrosisfactors(TNF)..TheproteinencodedbythisgeneisamemberoftheTNF-receptorsuperfamily.ThisreceptorisrequiredforgeneRationandlong-termmaintenanceofTcellimmunity.ItbindstoligandCD70,andplaysakeyroleinregulatingB-cellactivationandimmunoglobulinsynthesis.Theimmunesystemhasseveraleffectormechanismsatitsdisposaltofreethehostofpathogens.Becausetheseeffectormechanismscanalsodamagethehost,immuneresponsesshouldbetightlycontrolled.TNFRfamilymembersandtheirrespectiveTNFrelatedligandshavebeenimpliCatedintheregulationofsurvival,prolifeRation,differentiation,andmigRationduringadaptiveimmuneresponses.CD27isalymphoidcell-specificmemberoftheTNFRfamily.HumanandmurineT,B,andNKcellscanexpressCD27InHumans,CD27expressiondistinguishesbetweennaiveandeffector/memorystagesofperipheralBandTlymphocytes.OnBcells,CD27isonlyfoundafterBcellreceptor-inducedactivation.CD27-expressingBcellsfoundinHumanperipheralbloodallhaveundergonesomatichypermutationandthereforeCD27isconsideredaMarkerformemoryBcellsinHumans.RestingTcellsconstitutivelyexpressCD27,whiledifferentiationintoeffectorTcellsisaccompaniedbylossofCD27expression.

Source:

mAbstomurineCD27weregeneRatedinjectingamixtureofthemCD27tagexpresssingclonesAR0524andAR0530;HamsterspleencellsandSP2/0murinemyelomacellswerefused.

Product:

Eachvialcontains100ul1mg/mlpurifiedmonoclonalantibodyinPBScontaining0.09%sodiumazide.

Applications:

LG.8A6isusefulforflowcytometryand,immunoprecipitation.Optimalantibodydilutionshouldbedeterminedbytitration.IncombinationwithLG.3A10,itispossIBLetodevelopadoubledeterminantELISAtodetectsolubleMouseCD27.

Specificity:

LG.3A10andLG.8A6seeadifferentepitope.AcombinationcanthereforebedevelopedintoadoubledeterminantELISAtodetectsolubleMouseCD27.LG.3A10cross-reactwithHumanCD27,LG.8A6doesnot.

Storage:

Storeat4°C,orinsmallaliquotsat-20°C.

References:

1.Gravestein,L.A.,Nieland,J.D.,Kruisbeek,A.M.andBorst,J.Int.Immunol.7:551-557,1995.
2.Gravestein,L.,vanEwijk,W.,Ossendorp,F.andBorst,J.J.Exp.Med.184:675-685,1996.
3.Hendriks,J.,Gravestein,L.A.,Tesselaar,K.,vanLier,R.A.W.,Schumacher,T.N.M.,andBorst,J.NatureImmunol.1:433-440,2000.

Caution:

ThisproductisintendedFORRESEARCHUSEONLY,andFORTESTSINVITRO,notforuseindiagnosticortherapeuticproceduresinvolvinghumansoranimals.Thisproductcontainssodiumazide.Topreventformationoftoxicvapors,donotmixwithstrongacidicsolutions.Topreventformationofpotentiallyexplosivemetallicazidesinmetalplumbing,alwayswashintodrainwithcopiousquantitiesofwater.Thisdatasheetisasaccurateasreasonablyachievable,butNordic-MubioacceptsnoliABIlityforanyinaccuraciesoromissionsinthisinformation.

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